The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it Purpose of Catalase Test The morphologically similar Enterococcusor Streptococcus (catalase negative) and Staphylococcus (catalase positive) can be differentiated using the catalase test. Also valuable in differentiating aerobic and obligate anaer
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‏📌 Catalase test 🧫 is a biochemical test used to detect the presence of the catalase enzyme in bacteria. This enzyme breaks down hydrogen peroxide (H₂O₂) into water (H₂O) and oxygen (O₂), producing bubbles. 📍Procedure: 1. Place a drop of 3% hydrogen peroxide on a glass slide. 2. Add a bacterial colony using a sterile loop. 📍3. Observe the reaction: Positive ( ): Bubble formation, indicating the presence of catalase. Negative (-): No bubbles, indicating the absence of the enzymes 📍Significan
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